Different Media growth rate and pathogenicity of two species of entomopathogens found in Fiji

Ravneel Kumar, Ashmit Kumar


Two species of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae were collected in the field on larvae of the Black and Yellow Mud Dauber Sceliphron caementarium in the Instructional Agricultural Farm Complex of the Fiji National University’s College of Agriculture, Fisheries and Forestry. These fungi were cultured using different nutrient media to determine the type best suited for isolation and mass production. It was identified that both species responded well to growth in Egg Glucose Agar while a slower rate of mycelial formation occurred in Potato Dextrose Agar over a 7-day period under a photoperiod of 16 hours light and 8 hours of darkness at a relative humidity of 85%. Pathogenicity tests on Helicoverpa armigera larvae indicated that the isolate of Metarhizium anisopliae was more virulent than the strain of Beauveria bassiana with an LT50 time of 4 and 6 days respectively. B. bassiana has not previously been collected in the country while significant gaps still remain in the understanding of the potential for use of bio-control agents in agriculture locally. In this paper we attempt to determine the pathogenicity and growth rates of two entomopathogens using different media. 


Entomopathogens, Beauveria bassiana, Metarhizium anisipolae

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